Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 29;7:9651. doi: 10.1038/s41598-017-09884-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Application of FRI-NMR to study Ubiquitin-Gold nanorod interactions. (a) Overlay of the 2D [¹⁵N-¹H] HSQC spectra of Ubiquitin acquired at different gold nanorod (AuNR) to protein concentration ratios; (b) A transmission electron microscopy (TEM) image indicating the dimensions of nanorods; (c) A schematic illustration of the protein-gold nanorod interaction depicting the adsorption of the protein on the nanorod surface and the exchange of the adsorbed protein molecules with those in the bulk with a dissociation constant (K _D); (d) and (e) are the 1D traces along ¹H (ω₂) for the two residues (F4 and V26) shown magnified in (a) at different protein additions. The black vertical lines indicate the FRI-NMR estimates of chemical shifts. The high-resolution spectrum of free Ubiquitin is shown; (f), (g) are the corresponding plots of the change in the ¹H chemical shift with respect to free Ubiquitin for different additions of the protein estimated using the FRI-NMR method. The continuous blue line corresponds to the fit of the equation used for determining the indicated K _D values (see Section S4 of Supporting Information).