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. 2017 Aug 29;7:9605. doi: 10.1038/s41598-017-09867-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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tcf7l2 is required for regulation of insulin expression and exocrine pancreas development. Analysis of insulin in control embryos (A) and in tcf7l2 ^exI/exI mutants (A’) by in situ hybridization. Lateral views of the pancreatic area are shown with the anterior side to the right. The expression of insulin is significantly reduced in the mutants. (B–B”) Analysis of pancreatic islet in tcf7l2 ^exI/exI mutant in Tg(ins:dsRed) background. 2D projections of confocal Z-series images of DsRed expression in living Tg(ins:DsRed) embryos at 16 dpf. (B) wt; (B’) homozygous mutant. (C) Graphic presentation of the integrated density of fluorescence in the red channel in tcf7l2 ^exI/exI mutant and wild-type sib controls in Tg(ins:dsRed) at 16 dpf. (D) Quantification of the number of β cells during juvenile growth of tcf7l2 ^exI/exI and control siblings. (E–E’) Analysis of exocrine pancreas in tcf7l2 ^exI/exI mutant in Tg(ptf1a:dsRed), (E) wt and (E’) mutant at 16 dpf. (F) Graphic presentation of the integrated density of fluorescence in the red channel in tcf7l2 ^exI/exI mutant and wild-type sib controls in Tg(ela3l:Crimson) at 7 and 14 dpf. Data were obtained from 6 individuals per genotype, repeated in 2 different experiments. All reference to phenotypes was confirmed by genotyping. The integrated density was obtained using the Fiji software. Values represent the mean ± SEM. Asterisk above column indicate statistical differences among groups *p < 0.05.