Table 5.
Metabolite concentrations (µM) from T1 to T2 in the two groups.
| Responsive to therapy (R) | Not responsive to therapy (NR) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| T1 | T2 | Δ = T2 − T1 | Trend | T1 | T2 | Δ = T2 − T1 | Trend | |||
| lysoPC a C16:0 | 3.825 (2.320, 5.910) | 15.7 (10.3, 17.5) | * | 10.205 (2.77, 13.66) | ↑ | § | 2.780 (1.233, 4.902) | 4.020 (1.400, 6.572) | 0.250 (0.146, 1.648) | ↑ |
| lysoPC a C16:1 | 0.185 (0.124, 0.254) | 0.604 (0.470, 0.690) | * | 0.383 (0.118, 0.525) | ↑ | § | 0.116 (0.090, 0.160) | 0.158 (0.099, 0.238) | 0.012 (−0.002, 0.107) | ↑ |
| lysoPC a C17:0 | 0.139 (0.102, 0.191) | 0.312 (0.124, 0.376) | * | 0.131 (0.025, 0.199) | ↑ | 0.094 (0.059, 0.145) | 0.110 (0.080, 0.165) | 0.005 (−0.012, 0.064) | ↑ | |
| lysoPC a C18:0 | 0.902 (0.603, 1.650) | 3.220 (2.680, 4.400) | * | 2.054 (0.762, 3.330) | ↑ | § | 0.683 (0.382, 1.252) | 1.040 (0.494, 1.415) | 0.161 (0.071, 0.403) | ↑ |
| lysoPC a C18:1 | 1.170 (1.070, 1.970) | 6.090 (3.010, 6.770) | * | 4.160 (1.070, 5.320) | ↑ | § | 1.020 (0.709, 1.360) | 1.470 (1.170, 2.200) | 0.513 (0.242, 0.964) | ↑ |
| lysoPC a C18:2 | 1.215 (1.050, 1.380) | 6.075 (2.330, 8.060) | * | 3.455 (1.123, 6.732) | ↑ | § | 0.758 (0.572, 0.990) | 1.160 (0.916, 2.310) | 0.697 (0.212, 1.355) | ↑ |
| lysoPC a C20:3 | 0.222 (0.145, 0.333) | 0.474 (0.333, 0.834) | 0.245 (0.104, 0.600) | ↑ | § | 0.165 (0.116, 0.229) | 0.222 (0.147, 0.275) | 0.045 (0.022, 0.056) | ↑ | |
| lysoPC a C20:4 | 0.485 (0.359, 0.823) | 1.430 (0.800, 1.830) | 0.821 (0.262, 1.241) | ↑ | § | 0.478 (0.367, 0.688) | 0.456 (0.375, 0.985) | 0.059 (−0.006, 0.314) | ↑ | |
| PC aa C28:1 | 1.480 (1.280, 1.900) | 1.805 (1.460, 2.120) | * | 0.310 (−0.070, 0.520) | ↑ | 1.40 (1.105, 1.770) | 1.25 (1.083, 2.313) | 0.078 (−0.265, 0.430) | ↑ | |
| PC aa C32:3 | 0.948 (0.828, 1.130) | 1.110 (0.949, 1.420) | * | 0.152 (0.066, 0.270) | ↑ | 1.16 (0.816, 1.258) | 0.89 (0.850, 1.249) | 0.087 (0.349, 0.147) | ↑ | |
| PC aa C34:3 | 22.95 (17.9, 33.9) | 38.85 (24.9, 47.4) | * | 10.00 (0.700, 18.550) | ↑ | 23.50 (14.20, 29.00) | 23.70 (14.85, 34.25) | 1.300 (−1.725, 3.325) | ↑ | |
| PC aa C34:4 | 1.58 (1.29, 1.86) | 2.21 (1.38, 2.48) | * | 0.560 (0.190, 0.920) | ↑ | 1.48 (1.295, 1.693) | 1.160 (1.143, 1.600) | −0.141(−0.255, −0.023) | ↓ | |
| PC aa C36:1 | 55.8 (45.5, 70.9) | 81.75 (58.6, 95.4) | * | 20.15 (−0.500, 41.300) | ↑ | 50.90 (44.20, 55.40) | 63.60 (48.20, 82.25) | 6.500(1.250, 21.850) | ↑ | |
| PC aa C36:2 | 264.5 (182, 325) | 361.5 (324, 495) | * | 121 (9, 221) | ↑ | 222 (207, 223.75) | 298 (229.5, 313.25) | 74 (3.25, 102.25) | ↑ | |
| PC aa C36:3 | 124.5 (106, 147) | 188 (136, 227) | * | 76 (2, 97.6) | ↑ | 104 (88.60, 121) | 120 (110.25, 133.5) | 18.0 (−7.25, 19.80) | ↑ | |
| PC aa C38:0 | 1.630 (1.250, 2.080) | 1.745 (1.380, 2.730) | * | 0.340 (−0.140, 0.802) | ↑ | § | 2.010 (1.978, 2.237) | 1.670 (1.585, 1.778) | −0.440 (−0.548,−0.245) | ↓ |
| PC aa C38:1 | 0.601 (0.353, 0.833) | 0.661 (0.408, 0.740) | 0.079(−0.021, 0.259) | ↑ | § | 0.602 (0.385, 1.107) | 0.417 (0.347, 0.532) | −0.097 (−0.938, 0.003) | ↓ | |
| PC aa C38:3 | 28.8 (23.4, 41.2) | 36.750 (29.200, 47.700) | * | 9.25 (−1.000, 11.900) | ↑ | § | 28.30 (16.60, 31.175) | 25.30 (18.75, 30.025) | −0.90 (−6.300, 2.200) | ↓ |
| PC aa C38:5 | 37.8 (29.4, 49.2) | 47.85 (44.2, 51.9) | * | 13.85 (2.9, 19.4) | ↑ | § | 49.6 (41.225, 52.9) | 38.7 (30.7, 47.025) | −7.100 (−13.450, 2.175) | ↓ |
| PC aa C38:6 | 78.45 (51.6, 90.4) | 88.15 (70.8, 101) | * | 15.7 (5.9, 33.5) | ↑ | § | 69.8 (57.5, 91.2) | 62.2 (57.175, 77.9) | −11.30 (−17.85, −0.825) | ↓ |
| PC aa C40:4 | 1.655 (1.410, 2.020) | 2.115 (1.750, 2.630) | 0.39 (−0.13, 0.860) | ↑ | § | 1.840 (1.250, 2.473) | 1.590 (1.478, 2.003) | −0.310 (−0.540, 0.253) | ↓ | |
| PC aa C40:5 | 5.760 (3.700, 7.180) | 6.670 (6.220, 7.350) | * | 1.515 (−0.26, 3.08) | ↑ | § | 5.900 (4.890, 7.527) | 5.500 (4.575, 6.715) | −0.680 (−1.485, 0.512) | ↓ |
| PC aa C40:6 | 19.45 (15.2, 20.4) | 23.8 (17.7, 25.4) | * | 5.450 (2.500, 10.600) | ↑ | § | 19.4 (14.25, 23.725) | 19.4 (16.025, 19.8) | 0.000 (−3.775, 0.900) | // |
| PC aa C42:2 | 0.122 (0.101, 0.150) | 0.154 (0.121, 0.176) | * | 0.037 (−0.017, 0.067) | ↑ | 0.110 (0.089, 0.158) | 0.111 (0.102, 0.116) | −0.008 (−0.039, 0.015) | ↓ | |
| PC aa C42:4 | 0.085 (0.065, 0.112) | 0.105 (0.077, 0.151) | * | 0.012 (0.002, 0.037) | ↑ | 0.086 (0.080, 0.127) | 0.079 (0.075, 0.110) | −0.004 (−0.042, 0.018) | ↓ | |
| PC ae C34:2 | 13.4 (11, 19.1) | 15.3 (11.1, 23.8) | * | 2.80 (0.000, 6.230) | ↑ | 14.70 (11.650, 16.200) | 11.80 (11.025, 16.525) | −0.300 (−2.625, 1.212) | ↓ | |
| PC ae C34:3 | 6.205 (4.340, 7.470) | 6.575 (4.900, 9.800) | 0.070 (−0.600, 2.500) | ↑ | § | 6.760 (4.633, 8.373) | 4.490 (3.763, 6.342) | −1.570 (−3.197,−0.070) | ↓ | |
| PC ae C36:2 | 16.8 (14.6, 20.9) | 21.9 (15.2, 27.6) | * | 4.150 (0.400, 9.700) | ↑ | 18.20 (15.425, 21.150) | 17.20 (15.550, 24.925) | 2.60 (−1.825, 3.210) | ↑ | |
| PC ae C36:3 | 6.580 (5.270, 8.760) | 7.065 (6.210, 12.200) | * | 1.140 (−0.260, 2.800) | ↑ | § | 6.490 (6.460, 7.298) | 5.680 (5.215, 6.982) | −0.930 (−1.225, 0.212) | ↓ |
| PC ae C36:4 | 15.7 (13.4, 18.2) | 16.85 (13.2, 18.7) | 0.950 (−0.600, 3.650) | ↑ | § | 15.7 (14.675, 18.3) | 13.2 (12.225, 14.1) | −2.500 (−5.675,−1.700) | ↓ | |
| PC ae C36:5 | 9.88 (8.99, 11.9) | 11.1 (9.99, 12.2) | 0.920 (−1.200, 3.210) | ↑ | § | 10.2 (8.402, 14.125) | 8.470 (7.172, 10.372) | −3.700 (−3.850,−0.845) | ↓ | |
| PC ae C38:0 | 1.265 (1.120, 1.350) | 1.415 (1.280, 1.620) | * | 0.185 (0.100, 0.590) | ↑ | § | 1.190 (1.080, 1.395) | 0.970 (0.954, 1.238) | −0.110 (−0.199,−0.084) | ↓ |
| PC ae C38:1 | 0.407 (0.276, 0.614) | 0.649 (0.518, 1.020) | * | 0.162 (0.075, 0.387) | ↑ | 0.596 (0.248, 0.725) | 0.690 (0.582, 0.870) | 0.158 (−0.011, 0.250) | ↑ | |
| PC ae C38:2 | 1.725 (1.530, 2.040) | 1.845 (1.400, 2.730) | * | 0.360 (−0.040, 0.746) | ↑ | 1.860 (1.763, 2.013) | 1.910 (1.845, 2.113) | −0.030 (−0.088, 0.335) | ↓ | |
| PC ae C38:3 | 3.340 (2.760, 3.960) | 4.040 (2.960, 4.480) | 0.800 (−0.570, 1.290) | ↑ | § | 3.160 (2.530, 4.058) | 2.920 (2.353, 3.075) | −0.590 (−1.000,−0.198) | ↓ | |
| PC ae C38:4 | 11.75 (7.42, 14.2) | 10.71 (9.1, 14.1) | 0.350 (−0.800, 2.400) | ↑ | § | 14.1 (11.6, 15.15) | 10 (8.668, 12.1) | −2.540 (−4.033,−1.975) | ↓ | |
| PC ae C38:5 | 12.9 (11.4, 14.4) | 13.3 (10.2, 17.4) | 1.750 (−1.000, 4.400) | ↑ | § | 16.2 (13.9, 18.875) | 12.9 (10.725, 13.8) | −4.100 (−5.880,−3.000) | ↓ | |
| PC ae C38:6 | 4.910 (4.050, 5.720) | 4.720 (4.280, 7.240) | 0.685 (−0.100, 1.390) | ↑ | § | 5.270 (4.880, 6.490) | 3.940 (3.760, 4.133) | −1.760 (−2.057,−1.080) | ↓ | |
| PC ae C40:2 | 1.010 (0.851, 1.140) | 1.215 (1.060, 1.460) | * | 0.219 (0.120, 0.310) | ↑ | § | 1.180 (1.000, 1.335) | 1.010 (0.933, 1.275) | −0.120 (−0.377,−0.067) | ↓ |
| PC ae C40:5 | 2.040 (1.770, 2.370) | 2.405 (1.600, 2.700) | 0.390 (−0.100, 0.860) | ↑ | § | 2.660 (2.395, 2.767) | 2.110 (1.822, 2.190) | −0.700 (−0.935,−0.298) | ↓ | |
| PC ae C40:6 | 2.750 (2.390, 3.550) | 2.840 (2.640, 3.910) | * | 0.565 (0.220, 0.840) | ↑ | § | 3.020 (2.740, 3.775) | 2.570 (2.252, 2.865) | −0.410 (−1.168,−0.338) | ↓ |
| PC ae C42:2 | 0.223 (0.188, 0.259) | 0.250 (0.222, 0.294) | * | 0.219 (0.120, 0.310) | ↑ | 0.258 (0.195, 0.313) | 0.265 (0.209, 0.295) | −0.036 (−0.058, 0.041) | ↓ | |
| PC ae C42:3 | 0.339 (0.243, 0.395) | 0.395 (0.256, 0.512) | * | 0.078 (0.004, 0.140) | ↑ | § | 0.286 (0.266, 0.349) | 0.331 (0.261, 0.362) | 0.002 (−0.053, 0.040) | // |
| PC ae C42:4 | 0.272 (0.217, 0.415) | 0.300 (0.242, 0.482) | 0.036 (0.009, 0.090) | ↑ | § | 0.316 (0.272, 0.410) | 0.251 (0.216, 0.297) | −0.060 (−0.134,−0.005) | ↓ | |
| PC ae C42:5 | 0.875 (0.693, 0.944) | 0.984 (0.579, 1.160) | 0.066 (−0.146, 0.182) | ↑ | § | 1.020 (0.956, 1.293) | 0.887 (0.791, 1.051) | −0.169 (−0.365,−0.074) | ↓ | |
| PC ae C44:5 | 0.421 (0.302, 0.464) | 0.430 (0.231, 0.535) | −0.009 (−0.075, 0.154) | ↓ | § | 0.487 (0.438, 0.639) | 0.376 (0.323, 0.492) | −0.111 (−0.149,−0.090) | ↓ | |
| PC ae C44:6 | 0.319 (0.234, 0.345) | 0.342 (0.247, 0.435) | 0.053 (−0.015, 0.105) | ↑ | § | 0.405 (0.354, 0.517) | 0.304 (0.259, 0.361) | −0.126 (−0.177,−0.031) | ↓ | |
| SM (OH) C14:1 | 2.295 (1.830, 3.050) | 3.065 (2.220, 3.380) | * | 0.425(0.160, 0.890) | ↑ | 2.660 (2.023, 2.830) | 2.210 (2.152, 3.325) | 0.000 (−0.642, 0.352) | // | |
| SM (OH) C16:1 | 0.948 (0.761, 1.260) | 1.300 (1.010, 1.390) | 0.250 (0.041, 0.416) | ↑ | 1.170 (0.998, 1.405) | 1.030 (0.849, 1.293) | −0.240 (−0.353, 0.054) | ↓ | ||
| SM (OH) C22:1 | 0.731 (0.684, 0.915) | 0.837 (0.727, 1.140) | * | 0.082(−0.004, 0.289) | ↑ | 0.738 (0.574, 1.065) | 0.732 (0.516, 0.916) | −0.130 (−0.258, 0.146) | ↓ | |
| SM (OH) C24:1 | 0.071 (0.053, 0.098) | 0.077 (0.052, 0.098) | 0.003 (−0.011, 0.023) | ↑ | § | 0.065 (0.054, 0.095) | 0.054 (0.041, 0.069) | −0.015 (−0.033,−0.010) | ↓ | |
| SM C16:0 | 35.95 (31, 39.9) | 48.95 (38.7, 54.9) | * | 13.3 (3.8, 18.3) | ↑ | § | 42.9 (30.475, 48.575) | 40.6 (37.9, 45.275) | −0.900 (−6.175, 6.100) | ↓ |
| SM C16:1 | 5.570 (5.100, 6.520) | 6.815 (5.710, 8.650) | * | 1.260 (0.220, 3.190) | ↑ | § | 6.610 (5.390, 7.965) | 6.410 (5.200, 7.570) | 0.060 (−1.385, 0.643) | ↑ |
| SM C18:0 | 6.245 (5.380, 7.030) | 7.385 (6.880, 8.770) | * | 1.300 (0.450, 2.620) | ↑ | § | 6.010 (5.343, 9.427) | 6.430 (4.728, 6.750) | −1.580 (−3.072, 0.520) | ↓ |
| SM C18:1 | 3.020 (2.890, 3.700) | 3.585 (2.950, 4.210) | * | 0.525 (0.200, 0.840) | ↑ | § | 3.560 (2.887, 4.277) | 2.960 (2.325, 3.502) | −0.880 (−1.333, 0.065) | // |
| SM C20:2 | 0.127 (0.108, 0.142) | 0.169 (0.106, 0.258) | * | 0.045 (0.005, 0.116) | ↑ | 0.168 (0.128, 0.182) | 0.117 (0.107, 0.199) | 0.008 (−0.041, 0.044) | ↑ | |
| SM C24:0 | 1.050 (0.838, 1.380) | 1.235 (1.020, 1.560) | * | 0.158 (0.050, 0.465) | ↑ | 1.000 (0.747, 1.370) | 0.954 (0.882, 1.115) | −0.056 (−0.247, 0.145) | ↓ | |
| SM C24:1 | 3.410 (2.740, 4.310) | 4.885 (3.580, 5.310) | * | 0.565 (0.050, 2.420) | ↑ | 4.060 (2.388, 5.902) | 3.680 (3.293, 4.188) | −0.580 (−1.877, 0.998) | ↓ | |
| Arginine | 42.25 (29.2, 58.2) | 64 (56.1, 86) | * | 23.15 (3.500, 49.20) | ↑ | 40.70 (31.750, 59.850) | 69.20 (51.125, 86.200) | 25.10 (10.425, 54.075) | ↑ | |
| Histidine | 64.2 (51, 72.7) | 47.1 (37.1, 56.4) | * | −15.55 (−24.3, −2.4) | ↓ | 64.70 (51.525, 80.650) | 61.30 (56.500, 91.850) | 1.00 (−3.750, 7.375) | ↑ | |
| Lysine | 114.5 (103, 141) | 161 (135, 241) | * | 49.95 (6.0, 130) | ↑ | 146 (93.925, 271.750) | 190 (155.250, 217.750) | 55 (38.825, 81.575) | ↑ | |
| Ornitine | 23.65 (20.1, 31.1) | 58.95 (31.2, 84.4) | * | 12.60 (−2.70, 65.10) | ↑ | 31.30 (22.625, 42.875) | 69.40 (47.675, 92.650) | 34.00 (15.250, 76.493) | ↑ | |
| Serine | 41.05 (33.5, 50.3) | 63.1 (55.2, 84.4) | * | 22.30 (5.10, 29.80) | ↑ | 43.60 (38.275, 56.375) | 66.60 (54.800, 81.300) | 26.30 (7.900, 48.300) | ↑ | |
| Threonine | 56.2 (37.4, 68.7) | 78.35 (60, 89.6) | * | 24.70 (−3.80, 43.20) | ↑ | 53.70 (42.550, 74.100) | 80.40 (72.200, 102.300) | 19.80 (15.625, 35.325) | ↑ | |
| Trptophan | 16.25 (11.9, 21.6) | 27.6 (20.5, 35.1) | * | 9.335 (0.200, 19.10) | ↑ | 13.50 (9.720, 30.275) | 22.20 (21.875, 70.950) | 14.88 (4.050, 28.010) | ↑ | |
| Tyrosine | 40.2 (32.9, 45.9) | 50.95 (37.4, 62.4) | * | 9.05 (−4.900, 31.100) | ↑ | 52.00 (36.675, 62.500) | 48.10 (37.050, 90.875) | 2.80 (−2.475, 28.450) | ↑ | |
| Creatinine | 91.95 (64.7, 134) | 74 (55.9, 98.1) | * | −14.65 (−35.90, −7.0) | ↓ | 92.40 (82.475, 132.250) | 106 (77.225, 168.500) | 1.50 (−9.450, 19.60) | ↑ | |
| Met SO | 0.552 (0.001, 0.735) | 0.800 (0.591, 1.190) | * | 0.220 (−0.062, 0.859) | ↑ | 0.905 (0.116, 1.423) | 1.380 (0.227, 2.768) | 0.000 (−0.075, 2.196) | ↓ | |
| Taurine | 27.15 (14.6, 36.7) | 15.7 (10.7, 20.3) | * | −10.55 (−13.40, −4.0) | ↑ | 38.60 (30.125, 40.550) | 23.10 (13.900, 29.725) | −14.20 (−25.950, −7.400) | ↓ | |
| Kynurenine | 3.610 (2.530, 4.850) | 3.325 (2.630, 6.450) | 0.215 (−0.630, 1.780) | ↑ | § | 4.110 (2.848, 4.633) | 6.010 (4.840, 9.938) | 1.520 (1.380, 5.632) | ↑ | |
Significant differences between T1 and T2 are marked with *(Wilcoxon sign-rank test p < 0.05), §marks differences in the delta between R and NR (Wilcoxon rank-sum test p < 0.05). Metabolite abundance changed significantly from T1 to T2 in the NR group.