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. 2017 Aug 29;7:9644. doi: 10.1038/s41598-017-09433-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Volume status, hemodynamics, urine output and body weight during the fluid intervention (“Fluid”) and thirsting (“Zero”). Time course of fluid load (a), intracellular volume (b), extracellular volume (c), total body volume (d), relative fluid load (e), hemodynamics (f), urinary excretion (g) and body weight (h). Values are depicted as means ± standard errors of the means. Significant differences were determined by Student’s t-test and are marked (black stars) at the 0.05 probability level. U marks time point of urinating in an upright position.