Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 29;7:9622. doi: 10.1038/s41598-017-08985-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Performance of CelA and Cel7A on Avicel and DACS at 50 °C. CelA and Cel7A were incubated with Avicel and DACS at 50 °C for 24 and 96 h. β-glucosidase was added to each of these incubations at 5% total protein. Total enzyme doses ranged from 2 to 48 mg/g and glucose concentrations were measured by HPLC. (a,b) show the relative performance of CelA and Cel7A on these two substrates during 96 h incubations. This demonstrates that CelA is considerably better on Avicel than Cel7A even when tested at the temperature optimum of Cel7A. (c and d) show the 24 h and 96 h incubations for CelA and Cel7A, respectively, on DACS plotted as a function of enzyme x time. Model fits to these data are shown here – see Table 6 for parameter values. This demonstrates that the deviation in Et relationship is similar for CelA and Cel7A on DACS when tested at 50 °C.