. 2017 Aug 29;7:9622. doi: 10.1038/s41598-017-08985-w

Table 2.

CelA and CTec2 were tested on DACS, APCS, CFCS, and Avicel and the kinetic parameters from these model fits are shown.

Substrate	Enzyme	k _s (h⁻¹)	K_L (g/L)	k _i (×10⁻² h⁻¹)	t_1/2 (h)	t_1/2 95% CI (h)
DACS	Cb broth	54^a	1.1	4.4	16	13–18
DACS	CTec2	23 ± 3	100^a	0.3	206	54-
APCS	Cb broth	55 ± 7	100^a	2.2^b	31	—
APCS	CTec2	59 ± 54	100^a	3.0	23	7–142
CFCS	Cb broth	54 ± 2	100^a	2.2^b	31	—
CFCS	CTec2	20 ± 2	100^a	0	n/a	169-
Avicel	Cb broth	47 ± 4	100^a	2.2^b	31	—
Avicel	CTec2	6 ± 1	100^a	1.1	65	49–92

The model was fit to each progress curve shown in Fig. S3 by varying k _i and k _s or K_L. Values shown are best fit values and either their associated standard deviations or 95% confidence intervals.

^aValue was fixed while estimating values for remaining parameters.

^bValue was estimated using a global fit to CelA data on CFCS, APCS and Avicel.