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. 2017 Aug 29;7:9846. doi: 10.1038/s41598-017-10134-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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COL23A1 was down-regulated by small interfering RNA (siRNA) in ccRCC cell lines. (A,B) Relative COL23A1 mRNA levels in (A) 786-O and (B) A-498 cells that were transfected with siRNAs (#1, #2, #3) compared with siNC respectively were analyzed using qRT-qPCR. Error bars are ± SD (n = 3). (C) Results of western blot analysis showed the COL23A1 protein level of 786-O cells that were transfected with siRNAs (#1, #2, #3) compared with siNC, and the histogram right was quantification of the western blot analysis results. Error bars are ± SD (n = 3). (D) Results of western blot analysis showed the COL23A1 protein level of A-498 cells that were transfected with siRNAs (#1, #2, #3) compared with siNC, and the histogram right was quantification of the western blot analysis results. Error bars are ± SD (n = 3).