Figure 1.

Comparison between confocal microscopy scans from two different studies. Synapsin immunolabeling of the brain of a 20-day old (a) and a 35-day old (b) honeybee worker, Apis mellifera. (a,b) Single frontal confocal section of the olfactory lip region showing the distribution of anti-synapsin labeled boutons. Each yellow box indicates a volume of 10 µm × 10 µm × 10 µm. (a) Detail view from Fig. 5D from Peng and Yang¹⁷ (the yellow box was added). (b) Detail view from Fig. 1b from Groh et al.¹⁰. In both cases the lip regions contain synapsin-positive boutons of similar size, distribution and density. However, automated counting in Peng and Yang¹⁷, on average, revealed 1.9 boutons per 1,000 µm³, whereas visually guided counts by Groh et al.¹⁰ quantified 32.9 MG per 1,000 µm³. Scale bar: A: 25 µm (also applies to B).