Table 1.
Protocol of Immunization of mouse M4 with p13Bri for the Production of Conformational Antibodies Cross-reacting to Oligomeric Forms of Proteins and Peptides found in Neurodegenerative Diseases.
| Date of Inoculation (in days) | p13Bri Antigen Amount (μg/animal) | Antigen to Adjuvant Ratio* | Route of Inoculation | Identification of Bleed | Date of Bleed (in days) |
|---|---|---|---|---|---|
| — | NONE | n/a | NONE | Pre-Immune T0 | −7 |
| 0 | 50 | 4:1 | s.c | — | — |
| 14 | 50 | 4:1 | s.c | — | — |
| — | — | — | — | T1 | 21 |
| 28 | 20 | 9:1 | s.c | — | — |
| — | — | — | — | T2 | 35 |
| 49 | 20 | 9:1 | s.c | — | — |
| — | — | — | — | T3 | 56 |
| 69 | 20 | 9:1 | s.c | — | — |
| — | — | — | — | T4 | 76 |
| 91 | 20 | 9:1 | s.c | — | — |
| — | — | — | — | T5 | 98 |
| 119 | 20 | 9:1 | s.c | — | — |
| — | — | — | — | T6 | 126 |
| 165 | 10 | no adjuvant | i.v | — | — |
| 169 | Splenocytes fused to SP2/0-IL6** | — | — | TTB M4 | 169 |
*Sigma adjuvant system SC: Subcutaneous; IV: Intravenous; **Fusion Partner; TTB: Terminal bleeding.