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. 2017 Aug 29;7:9723. doi: 10.1038/s41598-017-10097-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) pAMPK/AMPK and PGC1α immunoblotting and quantitation (cropped images; full lenght blots are included as Supplementary Data). (B) qRT-PCR of mTOR, AMPK and LKB1 mRNAs. Data were generated from eight independent experiments and values were referred to mRNA levels of control normoxic mice and expressed as mean ± SD (N, normoxic control; 2 H, 2-day hypoxia; 10 H, 10-day hypoxia). Immunoblotting and qRT-PCR data were subjected to ANOVA and Tukey test (n = 5, p < 0.05). Differences between 2 H and 10 H versus N were indicated with the * symbol.