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. 2017 Aug 29;7:9723. doi: 10.1038/s41598-017-10097-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of key metabolic enzymes in skeletal muscle of mice exposed to 2-day (2 H) and 10-day (10 H) hypoxia. Representative immunoblot images (cropped images; full lenght blots are included as Supplementary Data) and histograms of protein expression levels of fatty acid synthase (Fasn), isocitrate dehydrogenase 1 (Idh1), glutamine synthetase (Glns), glutathione synthetase (Gss), HIF prolyl hydroxylase 2 and 3 (Phd2 and Phd3), mitochondrial malate dehydrogenase (Mdh2) and succinate dehydrogenase A chain (Sdha) in 2 H and 10 H compared to normoxic controls (N). Data were normalized against the total amount of loaded proteins stained with Sypro Ruby. Statistical analysis was performed by ANOVA and Tukey test (n = 5, p < 0.05). *Significant difference between 2 H and 10 H versus N.