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. 2017 Aug 25;8:1618. doi: 10.3389/fmicb.2017.01618

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Copyright © 2017 Dydecka, Bloch, Rizvi, Perez, Nejman-Falenczyk, Topka, Gasior, Necel, Wegrzyn, Donaldson and Wegrzyn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Levels of transcripts of indicated genes of wild type phages λ and Φ24_B and their deletion mutants in the absence or presence of control plasmid [pUC18] or plasmid bearing the deleted orf63 [pSB_orf63], assessed by quantitative reverse RT-PCR analysis, aftser prophage induction with 1 mM H₂O₂ in E. coli C600 host at 30°C. Levels of transcripts corresponding to particular genes were determined at following times after induction: 80 (blue), 120 (red) or 160 (green) minutes in case of phages λ and λΔorf63 (A), and 120 (blue), 180 (red) or 200 (green) minutes in case of phages Φ24_B and Φ24_BΔorf63 (B). Results are presented as mean values from three independent experiments with error bars indicating SD.