Figure 4.

Sex hormone levels in CFD and HFHS male and female mice with- and without- supraphysiological sex hormone supplementation. CFD, control mice; HFHS, high fat/high sugar mice. (A) Serum levels of testosterone in male CFD and HFHS mice with- and without- testosterone supplementation. (B) Serum levels of 17β-estradiol in female CFD and HFHS mice with- and without 17β-estradiol supplementation. (C) Uterine horn/body weight ratio in female CFD and HFHS mice with- and without 17β-estradiol supplementation. UH/BW, Uterine horn/body weight. ^*Significantly higher than non-supplemented levels of 17β-estradiol (p = 0.0003) and uterine horn/body ratio (p = 0.01). Testosterone supplementation did not significantly increase serum testosterone levels in male CFD or HFHS mice (p = 0.13).