Figure 2.

Horizontally acquired genes shared by more of the four studied Enterobacteriaceae family species tend to be more conserved in additional species as well. (A) Box plot representing the number of species in which “rare” pangenes were found, outside of the four Enterobacteriaceae family studied species. For each species four boxes are given: red – “rare” pangenes that are not shared in any of the three other species; yellow—“rare” pangenes shared by one additional species; green—“rare” pangenes shared by two additional species; blue—“rare” pangenes shared by all species. Whisker length for each boxplot represents 1.5 IQR. Statistical significance of differences between the gene-loss groups according to a non-paired, one-sided Mann-Whitney-Wilcoxon test is denoted by: ^***P ≤ 0.001. The Y-axis is presented in logarithmic scale. (B) Bar plot depicting the percent of “rare” pangenes in each sharing group that are not conserved outside the four analyzed species (orange), or that are shared outside the four analyzed species (blue).