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. 2017 Aug 29;7:9570. doi: 10.1038/s41598-017-10075-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myristic acid conversion by B. subtilis CYP109B1 supported by different redox partners. Reactions were started by the addition of a mixture of NADPH and myristic acid and allowed to proceed for 120 min under the support of an NADPH regenerating system. (a) CYP109B1 (1 µM) conversion reactions were carried out in the presence of non-fused redox partners Fpr and FldA (black bars), Fpr and YkuN (white bars) or with different YkuN-Fpr fusion constructs (grey bars). Reductase (Fpr) and flavodoxin (FldA or YkuN) together with CYP109B1 were employed at respective ratios of 1:1:1, 1:10:1 and 4:4:1. Reactions conducted with YkuN-Fpr (YR) fusion constructs and CYP109B1 were carried out at a respective ratio of 4:1. YR indicates the linker-less YkuN-Fpr fusion construct, whereas linker designations P1 - P5 and G1 - G5 correspond to linker sequences (GGGGS)_n and ([E/L]PPPP)_n of different lengths (n = 1–5). The data presented are average values of 3–6 independent conversion reactions with indicated standard deviation. (b) Myristic acid conversion by CYP109B1 in the presence of different concentrations of selected fusion constructs or non-fused Fpr/YkuN. The ratio of non-fused redox partners was maintained at 1:1.