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. 2017 Aug 29;7:9570. doi: 10.1038/s41598-017-10075-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Influence of auxiliary YkuN on CYP109B1 catalysis driven by the different YkuN-Fpr fusion constructs. Myristic acid conversion with CYP109B1 (1 µM) was carried out in the presence of the different Ykun-Fpr fusion constructs either in the absence (- YkuN) or presence of additional YkuN (4 or 9 µM). In all cases reactions were started by the addition of a mixture of NADPH and myristic acid and allowed to proceed for 120 min under the support of an NADPH regenerating system. The Fpr/YkuN/CYP109B1 system reconstituted at 1:10:1 and 1:1:1 ratio, respectively achieved 96% and 60% conversion of myristic acid in 120 min.