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. 2017 Aug 29;7:9570. doi: 10.1038/s41598-017-10075-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Conversion of β-ionone by T. fusca YX CYP154E1 supported by selected fusion constructs. Reactions were carried out in the presence of 1 µM CYP154E1 and 4 µM of indicated fusion constructs. Control reactions with non-fused Fpr/YkuN were conducted with 1 µM CYP154E1 together with either 1 µM Fpr + 10 µM YkuN [1:10], or 4 µM Fpr + 4 µM YkuN [4:4]. In all cases the initial concentration of the substrate β-ionone was 2 mM. Reactions were started by the addition of NADP⁺ under support of an NADPH regenerating system and stopped after 1 h.