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. 2017 Aug 15;9(8):3586–3598.

Figure 4.

Figure 4

MiR-130a-3p inhibiting CXCL12 expression. (A) Binding sites between miR-130a-3p and CXCL12 3’UTR and 6 mutant sites in CXCL12 3’UTR-mut would not complementary pairing to miR-130a-3p which be used as control. (B) Dual-luciferase assay showed that wt-3’UTR+miR-130a-3p mimics had a remarkably lower luciferase activity when compared with other three groups (student t-test, **P<0.01). (C) qRT-PCR and (D) Western Blot demonstrated that CXCL12 level was remarkably decreased when miR-130a-3p was overexpressed (student t-test, **P<0.01). Each assay was conducted in triplicate and repeated three times. The data show the mean of triplicate repeats ± s.e.m.