Figure 3.
Inhibition of autophagy reduced vascularisation in calcitriol-treated skin flap. A. Neovascularization in the control, the calcitriol and the calcitriol+3MA groups by H&E staining. (Original magnification × 200). C. The histogram of percentages of MVDs in the control group and the calcitriol group were 18.60 ± 2.61/mm2 and 31.60 ± 4.67/mm2, and 24.01 ± 3.54/mm2 in the calcitriol+3MA group respectively. B. Immunohistochemistry for CD34 positive vessels in these three groups. D. The number of CD34 positive vessels/mm2 in the control group was 13.20 ± 3.89/mm2, in the calcitriol group was 24.67 ± 3.89/mm2 and 17.60 ± 2.30/mm2 in the calcitriol+3MA group, respectively. Values are expressed as the mean ± SEM, n = 6 per group. *P < 0.05 versus the control group; ΔP < 0.05 versus the calcitriol group.