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. 2017 Aug 15;9(8):3642–3653.

Figure 4.

Figure 4

The suppression of autophagy down-regulated the levels of VEGF mRNA/protein in calcitriol-treated skin flap. A. In situ hybridization for VEGF mRNA in all groups (original magnification × 200). B. VEGF expression in all groups through Immunohistochemistry (original magnification × 200). C. The integral absorbance (IA) value VEGF mRNA in the control, the calcitriol and the calcitrol+3MA groups was 49056.80 ± 8673.32, 96172.20 ± 20242.82 and 62606.40 ± 5883.64, respectively. D. The integral absorbance (IA) value of VEGF protein in the control group was 49833.06 ± 6258.63, in the calcitriol group was 75796.20 ± 6333.97, and 53823.80 ± 4076.39 in the calcitriol+3MA group. E. Protein expression of VEGF in these three groups by western blot analysis. F. Densitometry results of VEGF protein expression in all groups. Values are expressed as the mean ± SEM, n = 6 per group. *P < 0.05 versus the control group; ΔP < 0.05 versus the calcitriol group.