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. 2017 Aug 15;9(8):3816–3826.

Figure 5.

Figure 5

HGF up-regulates COX2 through the Akt/PI3K and p38 MAPK signaling pathways. A: HGF induces Akt and p38 MAPK phosphorylation in MDA-MB-231 and MCF-7 cells. Cells were serum-deprived for 24 h followed by treatment as indicated, and extracted proteins were analyzed by Western blot to assay the expression of Akt and p38 MAPK and phosphorylated Akt and p38 MAPK. B-D: The HGF-induced increase in the expression of the COX2 protein occurs via the Akt and p38 MAPK pathways. MDA-MB-231 and MCF-7 cells were serum-deprived for 24 h and then treated with signaling inhibitors for 1 h followed by 60 ng/ml HGF treatment for 15 min for p-Akt and p-p38 MAPK detection and 12 h for COX2 detection. (*, P<0.05 vs. the control group; #, P<0.05 vs. the HGF or HGF+DMSO groups; **, P<0.05 vs. the HGF+LY294002, HGF+SB202190, HGF or HGF+DMSO groups).