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. 2017 Aug 29;7:9609. doi: 10.1038/s41598-017-09805-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rAAV2/9 transduction in the spiral ganglion cells (SGCs) and organ of Corti 4 weeks after virus inoculation at P0–1. (a) Midmodiolar cross-sectional images show transduced SGCs in Rosenthal’s canal (RC) (CN, cochlear nerve). Samples were immunostained with anti-GFP (green) antibody and anti-TuJ1 (red) to label SGCs. (b,c”) Images in cross-sections of the apical (b–b”) and basal (c–c”) turns are high-magnification views of the regions marked with white dotted squares. (d) Percent of transduced SGCs in RC. Data are means ± SD from four sections in each of two ears. eGFP expression of SGCs in RC was greater in the apical turn than in the basal turn (*p < 0.05; **p < 0.005). (e) In a cross section confocal image, rAAV2/9 transduced some cells in the stria vascularis (SV). Images were immunostained with anti-GFP (green) antibody and phalloidin (red) to label filamentous actin.