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. 2017 Aug 29;7:9609. doi: 10.1038/s41598-017-09805-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of the Efficiency and Specificity of AAV Transduction. (a) Comparative transduction efficiency and specificity of rAAV2/1 and rAAV2/9 carrying a CMV-eGFP expression construct delivered to wild-type murine cochlea at P0–1 by intravenous injection. Higher resolution images in the apical turn, utricle and crista ampullaris (CA). Green represents eGFP expression and red is Myo7a for labelling of hair cells. Overlapping Myo7a and eGFP localization represents positive hair cell transduction. (b) The efficiency of viral transuction in IHCs was assessed in 400 µm segments in the apical and basal turns (rAAV2/1 [gray] and rAAV2/9 [black]).