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. 2017 Aug 29;7:9863. doi: 10.1038/s41598-017-10594-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic and molecular characterization of cassava srd mutant. (A) Normal foliage development of wild type (WT) and srd mutants and delay of storage root formation in srd mutants after three months of growth in the greenhouse using shoot culture; no obvious change in mature leaves at the end of the dark period after iodine staining were noted; by contrast, the young stem of the srd mutant showed markedly decreased starch accumulation in cortical parenchyma cells than in the WT. (B) Reduced growth of storage roots in the srd mutant in the greenhouse (left and upper-right panels) and field-grown (bottom right panel) plants using stem cuttings. (C) Verification of the T-DNA insert by PCR using the primer pair FP1/RP1 and FP1/RP2, and qRT-PCR using the primer pair G1qrt5’F/G1qrt5′R and following Western blot using a specific antibody directed against MeGWD1, where it was found that the T-DNA insert in the eighth exon of MeGWD1 led to reduced protein expression.