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. 2017 Aug 29;7:9584. doi: 10.1038/s41598-017-09489-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Biochemical analysis of Tga20 mice inoculated with in vivo vs. in vitro GSS-related samples. Biochemical analysis of brain homogenates from Tga20 mice inoculated with the in vitro misfolded P102L-129M rec-PrP and the in vivo isolate from a GSS affected patient. Representative mouse brain homogenates from animals inoculated with both in vitro and in vivo samples were digested with 12,5 µg/ml of PK and purified according to a special procedure detailed in the Methods. An indistinguishable atypical pattern characterized by multiple bands of PK-resistant PrP fragments was observed in both groups of inoculated Tga20 mice. Classical sheep scrapie was used as control to show classical pattern. 12B2 monoclonal antibody (1:2,500). Mw: Molecular weight. Samples were run in separate gels, as indicated by the blank space between them, and images were cropped to not show unrelated samples run in the same gels. Alignment of both cropped blots was done according to the molecular weight markers.