Table 1.
Inoculation of in vivo vs. in vitro GSS-related samples in two different transgenic mouse models.
| Animal model inoculated | PrP species expressed | Survival time of positive animals (dpi) (±SEM) | Attack rate | PrPres detection rate | PrPSc pattern | |
|---|---|---|---|---|---|---|
| Misfolded rec-PrP | Tg340 | Human (4x) | 438 – 670 | 0/12 (0%) | 0/12 a | — |
| GSS brain PrPSc | Tg340 | Human (4x) | 671 ± 71 | 0/12 (0%) | 0/12 | — |
| Misfolded rec-PrP | Tga20 | Mouse (8x) | 506 ± 30b | 9/10 (90%) | 9/10 | Atypical |
| GSS brain PrPSc | Tga20 | Mouse (8x) | 297 ± 37 | 10/10 (100%) | 10/10 | Atypical |
aMice were euthanized at different days post-inoculation with non prion-specific clinical sings: 438 (6), 440, 442 (3), 454 (2) and 540 dpi. The rest of the animals did not show clinical signs up to 670 dpi. None of the brain samples were positive by Western blot or immunohistochemistry (IHC).
bOne mouse showed intercurrent disease at 102 dpi and was PrPres negative by Western blot. This animal was not included to calculate the SEM or attack rate. One mouse was found dead at 354 dpi without previous clinical signs but clearly PrPres positive. The rest of the mice were euthanized at different days post-inoculation with clear signs of prion-related clinical disease: 372, 384, 501, 511 (2), 547, 582 and 649 (2) dpi.
SEM: Standard Error of the Mean. dpi: days post inoculation.