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. 2017 Aug 29;7:9858. doi: 10.1038/s41598-017-07501-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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I27* unfolds at 480pN in the majority of force-clamp MD simulations. (a) End-to-end distances as a function of time for all 100 simulations. Each horizontal panel comprises 20 simulations, 100 ns each, separated by vertical lines, resulting in 10 µs of cumulative simulation time. (b) Cumulative frequency graph representing the time of unfolding for 73 out of the 100 force-clamp MD simulations for which unfolding was observed.