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. 2017 Aug 29;7:9711. doi: 10.1038/s41598-017-09859-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Combined in-silico and chromatin immunoprecipitation (ChIP) identifies p53 down-stream DNA-binding sites (p53BS) in the DPYD gene. (A) Schematic representation of the approach for in silico screening for putative p53BS within the DPYD gene and 20 kb upstream and downstream of the gene. (B) Fold-enrichment over untreated control of the p53BS (R-0.84) and other binding sites predicted by the in-silico analysis in mouse liver DNA as detected by ChIP analysis following IV 5-FU (150 mg/kg) administration for 6 hr (N = 3). (NS = Not significant) (C) Validation of p53DBS enrichment when treated as in (B), binding to p21 promoter is used as positive control. (N = 3) P-values were determined by multiple t-test.