Figure 5.

Sexual dimorphism in the expression for CRY1, CRY2 and FMO3 proteins. The daily rhythms in expression of proteins for CRY1 (a and b), CRY2 (c and d), FMO3 (e and f) in the liver: blue diamonds and dashed lines – AL male mice; blue squares and solid lines – CR male mice, red triangles and dashed lines – AL female mice; red x and solid lines – CR female mice. For all panels graphs are double plotted. Light is on at ZT0 and off at ZT12. Asterisk (*) indicates statistically significant difference (p < 0.05) in protein levels between the sexes. Open bars represent light and black bars represent dark phase of the day. Food for CR group was provided at ZT14. At every time of the day 3 mice of each sex were used for each diet. The respective images from Western blot analysis are shown in Supplementary Figs S1 and S2.