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. 2017 Aug 29;7:9750. doi: 10.1038/s41598-017-09649-5

Figure 1.

Figure 1

Procuticle characteristics in D. pulex and D. longicephala. (A) STEM images of procuticle cross-sections. (i) D. pulex uninduced, scale-bar = 1 µm (ii) D. pulex induced, scale-bar = 1 µm (iii) D. longicephala uninduced, scale-bar = 1 µm iv) D. longicephala induced, scale-bar = 2 µm. (B) Procuticle thickness of the uninduced and the induced morphotype of D. pulex and D. longicephala. (Mann Whitney U-test, D. pulex: n (uninduced; induced) = 17, D. longicephala n (uninduced; induced) = 20; level of significance: *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.005). (C) Procuticle number of layers of the uninduced and the induced morphotype of D. pulex and D. longicephala. (Mann Whitney U-test, D. pulex: n (uninduced) = 13, n (induced) = 16; D. longicephala n(uninduced) = 21, n(induced) = 19; level of significance: *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.005) (D) Procuticle Young´s-modulus of D. pulex (left) and D. longicephala (right) in uninduced and induced form, measured on an AFM. (nested ANOVA, D. pulex: n (uninduced; induced) = 5 with min 770 measurements each; D. longicephala n (uninduced) = 6, n (induced) = 7 with min 770 measurements each; level of significance: *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.005).