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. 2017 Aug 29;7:9613. doi: 10.1038/s41598-017-10110-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of injection rate on spreading of drug solution. Temporal variations of the WF position through the tissue when the drug solution was injected at volume infusion rates of (a) 25 μL/min and (b) 100 μL/min. Insets are X-ray images of the injection region of the subcutaneous tissues. (c) Aspect ratios of depot formations in the injection and diffusion regions. (d) Comparison of the experimental results and theoretical predictions of normalized WF. Scale bar: 500 μm.