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. 2017 Aug 29;7:9715. doi: 10.1038/s41598-017-10136-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Interaction of LptC dimer and of LptC-LptA_m complex with the E. coli lipopolysaccharide. Representation of methyl ¹H and ¹³C combined CSP induced by LPS (0.8 mg/ml) in the isolated LptC dimer (a) or in the LptC-LptA_m complex (b). Gradient colors used for the display of the CSP values are shown. Residue in magenta disappeared upon LPS addition. For clarity only one LptA_m molecule of the LptC-LptA_m complex is represented. Residues of LptA_m colored in blue correspond to the perturbed area upon LptC interaction (as in red on Fig. 4e). (c) [¹H, ¹³C]-correlation spectrum of methyl-labeled and perdeuterated LptC in complex with unlabeled LptA_m with (red) or without (black) 2.1 mg/ml of LPS. (d) [¹H, ¹⁵N]-correlation spectrum of [²H, ¹⁵N]-labeled LptA_m in complex with unlabeled LptC without (green) or with (red) 2.1 mg/ml of LPS. The resonances assigned in the free form of LptA_m are indicated by a cross.