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. 2017 Aug 29;7:9826. doi: 10.1038/s41598-017-10377-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry assay showing apparent reduction in cytosolic calcium concentration in human DASMCs transfected with NCX1-cDNA. (a) Compared with the control and negative control groups, DASMCs transfected with NCX1-cDNA express higher levels of NCX1 mRNA. (b) Cropped blots showed that compared with the control and negative control groups, DASMCs transfected with NCX1-cDNA express higher levels of NCX1 protein. (c) Negative control (red) and siARHGAP26 (blue) groups are shown (n = 3). (d) Quantification of mean calcium fluorescence intensity showed an apparent reduction in cytosolic calcium concentration in DASMCs transfected with NCX1-cDNA. (e) Calcium imaging showed that after overexpression of NCX1 the fluorescence in the DASMCs which represent for the cytosolic calcium concentration was reduced compared to the negative control group. The scale bar is 25 μm. The error bars indicate means ± SD. *P < 0.05, **P < 0.01.