Table 1. Oligodeoxyribonucleotides used for PCR-mediated mutagenesis.
| Enzyme |
Residue |
Forward oligonucleotides |
| N.BstNBI | D389A | AACTTGAAGAAGTCATTGCTCTTCTTGAGGTATATCATGA |
| E392A | AGTCATTGACCTTCTTGCAGTATATCATGAGAAAAAGAAT | |
| E418A | AAATACTGTATTTGCCTGGCTTACGTGGAATGGCTTC | |
| D441A | AAACAACTTCGTTATTGCAGAAGAGTTACAACCAGTTA | |
| D456 | GCCGCAGGTAACCAGCCTGCCATGGAAATTATATATGAAG | |
| E469A | ACTTTATTGTTCTTGGTGCCGTAACAACTTCTAAGGGAGC | |
| E482A | AACCCAGTTTAAGATGGCGTCAGAACCAGTAACAAGGCAT | |
| PleI | D355A | GTTATACGAAGCCATTCAAGAGGTTTTTG |
| E358A | GAAGACATTCAAGCGGTTTTTGAGAAG | |
| E377A | ATTGATGCTTGCCTGGAATACATGG | |
| D399A | AAATTTGAAATTTGCGGATTTTGGAAGT | |
| D414A | GGAAATATGCCAGCTATAGTGTGTG | |
| E422A | AGTTGTCTGTAGCTGTCACAATGGC | |
| E440A | AATATGAAATGGCAGGTGAGCCAGTC | |
| MlyI | D359A | CTTAGATCTGATATTGAAGCTATTTTAGACGTCTTTGC |
| D363A | GATATTTTAGCCGTCTTTGCAAAAA | |
| E380A | GTTCCTTTATTCCTTGCATGGAATATATGG | |
| D403A | GGAACTTCATTGTAGCTTTAGATGGAAT | |
| D418A | CAGGTAAGAAGCCTGCTATAGAAATTGGT | |
| E430A | ATCTTTTTCATGCATTGTTGCAGTAA CTATGTCATCAGGG | |
| E444A | CTCAATTTAATATGGCGGGGTCTTCTGTT′ |
Both strands were synthesized and the forward sequences are shown in the 5′→3′ orientation. The targeted codons are underlined.