Table 1.

Kinetic parameters for the activity of wild-type mIDH2 (WT) and the K256Q and K413Q mutants.

Enzyme	K m (μM)	V max (μM s−1)	k cat (s−1)	k cat /K m (s−1 μM−1)
(Kinetic parameters for isocitrate)
WT	54.4 ± 5.6a	0.028 ± 0.001	5.60 ± 0.14	0.111 ± 0.011
K256Q	30.4 ± 2.3	0.013 ± 0.0002	2.66 ± 0.04	0.088 ± 0.008
K413Q	55.5 ± 7.1	0.023 ± 0.001	4.61 ± 0.15	0.083 ± 0.008
(Kinetic parameters for NADP+)
WT	33.7 ± 2.4	0.031 ± 0.0006	6.36 ± 0.14	0.186 ± 0.011
K256Q	14.8 ± 1.3	0.015 ± 0.0003	3.13 ± 0.13	0.205 ± 0.022
K413Q	112.2 ± 13.2	0.033 ± 0.001	7.69 ± 0.49	0.059 ± 0.001

^aValues are the means of four technically independent replicates and are displayed as the means ± SD.