Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 29;7:9815. doi: 10.1038/s41598-017-10168-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Decreased inflammation, inflammatory mediator production and apoptosis with 6-ECDCA pretreatment. (A and B) Representative images of immunostaining for (A) CD4 and (B) TNFα in renal sections from (a) sham, (b) I/R and (c) I/R + ECDCA groups (scale bar 50 μm). Note the tubulointerstitial localization of CD4⁺ and TNFa⁺ inflammatory cells. (C) Levels of mRNA for Tnfα, Il6 and Mcp1 in kidney tissue. n = 6 mice/group. (D and E) Blood levels of (D) TNFα and (E) MCP1. n = 6 mice/group. (F) Representative images of TUNEL staining on renal sections from (a) sham, (b) I/R and (c) I/R + ECDCA groups (scale bar 50 μm). Arrows indicate apoptotic bodies. (G) Quantitative analysis of positive TUNEL stained cells per high power field. n = 6 mice/group. (H) Levels of Bax, Caspase3 and Bcl2 mRNA in kidney tissue. n = 6 mice/group. Data are means ± SEM, one-way ANOVA with Bonferroni’s test. *p < 0.05. (I) Western blot analysis of ICAM and cleaved-caspase 3 protein levels in kidney samples of sham, I/R and I/R + ECDCA mice.