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. 2017 Aug 29;7:9815. doi: 10.1038/s41598-017-10168-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Attenuation of I/R-induced oxidative stress by 6-ECDCA treatment. At 24 h after treatment, (A) urinary H₂O₂, (B) urinary NGAL, (C) kidney MPO and (D) kidney MDA were analyzed. n = 6 mice/group. Data are means ± SEM, one-way ANOVA with Bonferroni’s test. *p < 0.05. (E and F) Representative images of immunostaining for (E) 4-HNE and (F) oxo-8 staining on renal sections from (a) sham, (b) I/R and (c) I/R + ECDCA groups (scale bar 50 μm). (G) 2′,7′-DCF staining on renal sections from (a) sham, (b) I/R and (c) I/R + ECDCA groups (scale bar 25 μm). (H–J) Quantitative analysis of (H) 4-HNE, (I) oxo-8 and (J) 2′,7′-DCF staining per high power field. n = 6 mice/group. Data are means ± SEM, one-way ANOVA with Bonferroni’s test. *p < 0.05.