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. 2017 Aug 29;7:9599. doi: 10.1038/s41598-017-09943-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of melatonin (M) on chronic stress-induced oxidative stress in testes. (A) ROS level relative to control group (n = 6). (B) GSH content in different groups (n = 6). (C) SOD content in different groups (n = 6). (D–E) The protein level of iNOS detected by Western blot, and relative level compared to the housekeeping molecule ACTB (n = 5). All data are presented as mean ± SEM. *P < 0.05 vs. control group; **P < 0.01 vs. control group; ***P < 0.001 vs. control group; ^#P < 0.05 vs. stress group; ^##P < 0.01 vs. stress group; ^###P < 0.001 vs. stress group. Control, non-stress treated with saline; Control+M, non-stress treated with melatonin; Stress, stress treated with saline; Stress+M, stress treated with melatonin.