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. 2017 Aug 29;7:9600. doi: 10.1038/s41598-017-08821-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of PrP^Sc, Iba1, and ferritin in hamster retinas. (a) Immunoreaction for PrP^Sc shows no reactivity in mock-treated control retinas (panel 1). PrP^Sc deposits are first evident at 49 dpi, and are localized to outer segments of photoreceptor cells, OPL, INL, IPL, and GCL (panel 2). Robust PrP^Sc deposits are seen throughout the retina at 75 dpi and in clinical samples (panels 3 & 4). (b) Immunoreaction for Iba1 shows minimal reactivity in RPE, OPL, and IPL in mock treated samples (panel 1). Prominent reactivity for Iba1 is first seen at 49 dpi (panel 2). Amoeboid microglia are evident throughout the retina, including the RPE cell layer in scrapie-infected samples (panels 3 & 4). Arrows indicating large cell bodies of activated, Iba1 + microglia at later time points (panels 3 & 4). (c) Immunoreaction for ferritin shows minimal reaction in mock-treated samples (panel 1). Increased perinuclear and cytoplasmic immunoreactivity for ferritin is first evident at 49dpi, and is localized to RPE, OPL, INL, IPL, and GCL (panels 2–4). High magnification (60X) inserts in the upper right hand corner show Iba1 and ferritin immunoreactivity in the RPE cell layer in scrapie-infected samples. Abbreviations: GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer; RPE: Retinal pigment epithelium. Scale bars: 10 μm. (d) The increase in ferritin reactivity occurs subsequent to PrP^Sc accumulation, and coincides with Iba1 reactivity.