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. 2017 Aug 29;7:9791. doi: 10.1038/s41598-017-09952-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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: FMNL2/3 KO causes malformation of lysosomes. (a) Anti-LAMP1 staining visualizing lysosomal compartments, with co-staining by phalloidin to reveal overall cell morphology; merges on the right, as indicated (actin filaments in red, lysosomes green). Note the prominent difference in appearance of lysosomes in control NIH3T3 cells versus FMNL2/3 KO clones. (b) Cell images on the left: Representative examples for manual categorization of cellular lysosome morphologies, as indicated (“circular perinuclear”, most representative for lysosome appearance in NIH3T3 control fibroblasts, “small dispersed” as intermediate phenotype and “diffuse cloud” as most severe phenotype. Right panel: Bar chart illustrating results of manual categorization according to the color code depicted on the left, with stacked columns corresponding to arithmetic means and error bars to sem; n gives number of cells analyzed, statistical significance of differences was determined for parameter “diffuse cloud” (t-test). (c) Western blot illustrating comparable expression levels of LAMP1 in control versus FMNL2/3 KO cells. Tubulin validates equal loading.