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. 2017 Aug 29;7:9791. doi: 10.1038/s41598-017-09952-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Loss of FMNL2/3 does not affect Golgi reassembly from the ER. (a) Representative GM130-stainings of NIH3T3 control and FMNL2/3 KO cell lines not treated (“−BFA”) or treated with Brefeldin A (2,5 µg/ml; “+BFA”) for 30 min, followed by replacement of BFA-containing medium with regular growth medium (“washout”) for the times indicated. Note that prior to BFA treatment, the Golgi apparatus is less organized and less compact in both KO cell lines compared to control cells, consistent with the data shown above. (b) Quantitation of average number of Golgi fragments in different cell lines and conditions as indicated. Both KO clones were normalized to the average number of Golgi fragments in control cells (“−BFA” = before treatment); n is number of cells analyzed. Differences between the 60 min washout stainings and those of untreated cells (“−BFA”) were confirmed not to be statistically significant (t-test) in all cell lines, indicating complete recovery from BFA-treatment, irrespective of the presence of FMNL subfamily formins.