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. 2017 Aug 29;7:9857. doi: 10.1038/s41598-017-09886-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Changes in expression of rnaIII and spa by ajoene. Expression measured by reporter fusion based plate assay (a,b), Northern blot (c,d) and qRT-PCR (e,f). (a) Expression of rnaIII measured with rnaIII::lacZ reporter fusion. (b) Expression of spa measured with spa::lacZ reporter fusion. (c) Expression of rnaIII and (d) expression of spa with the addition of 1 μg/ml and 5 μg/ml of ajoene compared to controls with addition of DMSO or H₂O. The right panels in (c,d) show the loading controls of ethidium bromide stained total RNA. (e) qRT-PCR data of samples retrieved at exponential growth phase and, (f) at stationary growth phase for rnaIII (dark grey bars), spa (medium grey bars) and hla (light grey bars).