Figure 5.

Epithelial tissue factor (TF) plays a minor role in acid‐induced acute lung injury (ALI). Epithelial TF‐deficient and wild‐type (WT) mice were treated with HCl for 8 h. (A) TF mRNA expression of lung tissue was determined by qPCR, and is depicted as fold wild‐type control (n _{control TF +/+} = 5; n _{control TF Δmye} = 3; n _{HC l TF +/+} = 9; n _{HC l TF Δmye} = 10). (B, C) Activated factor X (FXa) generation (B) (n _{control TF +/+} = 4; n _{control TF Δmye} = 2; n _{HC l TF +/+} = 6; n _{HC l TF Δmye} = 4) and thrombin–antithrombin (TAT) complex (C) were measured in bronchoalveolar lavage fluid (BALF) (n _{control TF +/+} = 4; n _{control TF Δmye} = 1; n _{HC l TF +/+} = 9; n _{HC l TF Δmye} = 7). (D) Protein content in BALF samples (n _{control TF +/+} = 4; n _{control TF Δmye} = 2; n _{HC l TF +/+} = 10; n _{HC l TF Δmye} = 6). (E) Lung edema was determined by measuring the weight ratio of wet and dried whole lung tissue (n _{control TF Δmye} = 2; all others n = 5) (F–H) IL‐6 mRNA expression (F) (n _control = 4; n _{HC l TF +/+} = 5; n _{HC l TF Δmye} = 8) and IL‐6 protein levels in (G) total lung tissue (n _{control TF Δmye} = 1; all others n = 4) and (H) BALF (n _{control TF +/+} = 4; n _{control TF Δmye} = 1; n _{HC l TF +/+} = 6; n _{HC l TF Δmye} = 3). (I, J) Flow cytometric analysis of (I) neutrophils (CD45⁺ Ly6G⁺ F4/80⁻) and (J) erythrocytes (Ter119⁺) in the BALF of epithelial TF‐deficient and WT mice (n _{control TF +/+} = 4; n _{control TF Δmye} = 2; n _{HC l TF +/+} = 11; n _{HC l TF Δmye} = 8). For statistical analysis, the Mann–Whitney test was performed (A, B); ***P < 0.001. Littermate‐controlled experiments were performed. HPRT, hypoxanthine guanine phosphoribosyl transferase; ND, not detectable.