Figure 2.

Expression and localization of F4C in roots.

(a) Expression of FLA4‐citrin in the root meristem and early elongation zone. Transverse x,z‐projections through the (from bottom) quiescent center, cell division‐, transition‐ and elongation zone. The construct is maximally expressed in the epidermis of the division zone. During maturation the epidermal expression decreases and the expression in more central cell layers increases (scale bar in (a) = 100 μm).

(b) High resolution conventional confocal (left) and STED confocal (center) micrograph taken in the cell division zone (scale bar in (b) = 5 μm).

(c, d) (c) Partial overlap between FLA4‐citrin and RabF2a‐cerulean or (d) RabA1e‐cerulean in diffuse intracellular compartments (arrowheads) but not in the brightest RabF2a‐cerulean punctae (arrows). Scale bars in (c) and (d) = 5 μm.

(e) Treatment with 25 μM brefeldin A for different durations (scale bar in (e) = 10 μm).