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. 2017 Jun 13;91(4):613–630. doi: 10.1111/tpj.13591

Figure 3.

Figure 3

FLA4‐citrin is localized both at the plasma membrane and in the apoplast.

(a) FLA4‐citrin and FM4‐64.

(b) SKU5‐GFP and FM4‐64.

(c) PIP1;4‐YFP.

(d) Equivalent proportions (percentage of total) of the pellet after centrifugation at 300 g (P300) and the supernatant (SN) and pellet (P100k) after subsequent 100 000 g centrifugation, were loaded on gels and probed with antibodies against GFP, SKU5 and PIN2. The extraction was replicated with material previously exposed to partial plasmolysis by 500 mm mannitol. The asterisk on the blot exposed to a‐PIN2 antiserum indicates an unspecific band as previously noted (Abas et al., 2006).