Figure 4.

Localization FLA4‐citrin∆GPI.

(a) Requirement of the C‐terminal region for membrane association. Transgenic lines expression full‐length FLA4‐citrin (F4C) or C‐terminally truncated F4C (F4C∆GPI) are separated into high‐speed (100 000 g) supernatant (SN) and pellet (P). Cytosolic fructose‐1,6,‐bisphisphatase (cFBPase) is only detected in the soluble fraction.

(b) F4C∆GPI co‐localizes with ER‐targeted RFP (erRFP).

(c) Plasmolysis of full‐length and FLA4‐citrin∆GPI indicates residual secretion but no plasma membrane localization of F4C∆GPI (scale bar for (c, d) = 10 μm).

(d) After brefeldin A (BFA) treatment F4C∆GPI is partially localized to BFA body‐like structures (arrowheads).