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. 2017 Jun 13;91(4):613–630. doi: 10.1111/tpj.13591

Figure 5.

Figure 5

Normal localization of F4C depends on the N‐proximal region of the protein.

(a) Constructs lacking only the Fas1‐1 region (F4C∆Fas1‐1) or the Fas1‐1 and the entire PR1 and the N‐terminus of the Fas1‐2 region (F4C∆Fas1‐1∆PR1.6) are mis‐localized to different compartments.

(b) Co‐labelling of F4C∆Fas1‐1 and F4C∆Fas1‐1∆PR1.6 with erRFP.

(c) Co‐labelling of F4C∆Fas1‐1 and F4C∆Fas1‐1∆PR1.6 with RabA1e‐cerulean.

(d) Co‐labelling of F4C∆Fas1‐1 and F4C∆Fas1‐1∆PR1.6 with RabF2a‐cerulean.

(e) Co‐labelling of F4C∆Fas1‐1 and F4C∆Fas1‐1∆PR1.6 with FM4‐64 in partially plasmolyzed cells.