Figure 6.

N‐glycosylation of F4C.

(a) Treatment of oligomannosidic N‐glycans by Endo H and release of complex N‐glycans by PNF in the MUR1 wild‐type and the GDP‐L‐fucose defective mur1‐2 background. In mur1‐2 F4C becomes fully sensitive to PNF indicating fucosylation and additional N‐glycan processing.

(b) The F4C∆Fas1‐1 product is only partially sensitive to Endo‐H and PNF while the ER‐retained F4C∆Fas1‐1∆PR1.6 product is fully sensitive to both enzymes indicating that it exclusively contains oligomannosidic N‐glycans.