Figure 7.

The conserved N‐glycosylation site N207 is required for normal endoplasmic reticulum (ER) to Golgi transit.

(a) The substitution mutant F4C_N207Q shows strongly increased sensitivity to PNF indicating an increased ratio of oligomannosidic to fucosylated complex N‐glycans. A minor residual fraction of PNF resistant product is only detected when blots are over‐exposed.

(b) F4C_N207Q co‐localizes with reticulate ER structures (arrowheads) but does not accumulate in fusiform ER bodies (arrows).