Figure 8.

F4C carries AGP‐associated carbohydrate epitopes.

(a) A SDS‐PAGE gel of affinity‐purified F4C shows a protein doublet (arrow) at 91 kDa and a single protein band (asterisk) at around 53 kDa.

(b) Out of a variety of carbohydrate‐specific monoclonal antibodies only LM14 and JIM13 react with affinity‐purified F4C.

(c) Treatment of affinity‐purified F4C with O‐glycosidases removes reactivity with LM14.

(d) Both AGP antibodies react with heterogeneous fraction that is PNF sensitive only in the mur1‐2 background but not with the minor fraction that is generally PNF sensitive.

(e) LM14 reacts with full‐length F4C and with truncated F4∆Fas1‐1 but not with the ER‐retained F4C∆Fas1‐1∆PR1.6.